Probe details
Rfla729
Tested for in situ hybridization.
|
| Accession no. |
pB-557 |
| Full name (Alm et al.) |
S-*-Rfla-0729-a-A-18 |
| Categories |
|
| Specificity |
Ruminococcus albus, R. flavefaciens |
| Taxonomy |
Ruminococcus;
Oscillospiraceae;
Eubacteriales;
Clostridia;
Bacillota;
Bacillati;
Bacteria;
cellular organisms
|
| Sequence |
5'-AAA GCC CAG TAA GCC GCC-3' |
| Length [nt] |
18 |
| G+C content [%] |
61 |
| Target rRNA |
16S |
| Position |
729-746 |
| Formamide |
20 |
| Check specificity/coverage |
SILVA TestProbe
|
| References |
Extensive set of 16S rRNA-based probes for detection of bacteria in human feces. Harmsen HJ, Raangs GC, He T, Degener JE, Welling GW. Applied and environmental microbiology. 2002. Pubmed: 12039758
Design and validation of 16S rRNA probes to enumerate members of the Clostridium leptum subgroup in human faecal microbiota. Lay C, Sutren M, Rochet V, Saunier K, Doré J, Rigottier-Gois L. Environmental microbiology. 2005. Pubmed: 15946290
|
| Remarks |
15 min treatment with 10 µl of 1 mg lysozyme per ml in 100 mM Tris-HCl (pH 8.5). More competitors: 3. AAAGCCCAGCAAGCCGCC and 4. TCAGTCCAGTAAGCCGCC. |